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Figure 4

From: Identification of a Histidine Metal Ligand in the argE-Encoded N-Acetyl-L-Ornithine Deacetylase from Escherichia coli

Figure 4

UV–vis absorption spectra of 1 mM samples of (A) WT ArgE in the presence of 1 equivalent of Co(II), (B) WT ArgE in the presence of 2 equivalents of Co(II), (C) H355A ArgE in the presence of 1 equivalent of Co(II), and (D) H355A ArgE in the presence of 2 equivalents of Co(II). (Reaction conditions: 25°C in 50 mM HEPES buffer, pH 7.5, and 150 mM KCl).

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