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Table 1 Comparison of the average (mean ± SD) yield and ratio of A 260nm /A 280nm of RNA Isolate using 3 different methods; LeukoLOCK™, Tempus™, and TRIzol® reagent

From: Isolation of RNA from equine peripheral blood cells: comparison of methods

Methods

LeukoLOCK(n=10)

Tempus(n=10)

TRIzol® reagent (n=10)

A 260nm /A 280nm

2.06 ± 0.05

1.97 ± 0.06

1.98 ± 0.06

Yield (μg/ml)

0.99 ± 0.07

1.08 ± 0.09

3.78 ± 0.12

  1. The ratios of A260nm/A280nm for the 3 RNA extracts from the 3 different methods were > 1.80, indicating high RNA purity without contamination by protein and other organic molecules.