Figure 4

Relative expression levels of IL-6 after Dnase treatment (n=5). A: RNA Samples extracted using Method 1 (LeukoLOCK™ total RNA isolation system); B: RNA samples extracted using Method 3 (TRIzol® reagent) and then treated with Dnase; C: RNA samples extracted using Method 3 (TRIzol® reagent). Sample A was control. After sample C was treated with Dnase, the relative expression level of IL-6 (sample B) was reduced to 1.20 ± 0.22, indicating that DNA contamination in sample C was eliminated.