Figure 3

Relative expression of IL-6 in the RNA extracts from 3 different isolation methods (n=5). A: RNA extract using Method 1 (LeukoLOCK™ total RNA isolation system); B: RNA extract using Method 2 (Tempus™ blood RNA tube with ABI PRISM® 6100 Nucleic Acid PrepStation); C: RNA extract using Method 3 (TRIzol® reagent). Sample A was control. All the samples were extracted from the same host at the same time. The relative expression level of IL-6 in Sample B was 1.11±0.18, and that of C was 2.93±0.39, suggesting that there was a trace of DNA contamination in sample C, but not in B.