Table 3 Studies assessing mRNA retrieval from HPV-DNA positive cervical samples using currently available detection assays
Author | Year | Detection technique | Automation | HPV types included in assay | E6/E7 mRNA detection rate |
|---|---|---|---|---|---|
Molden | 2005 | Pre-Tec HPV Proofer NASBA | Automated | 16 18 31 33 45 | 98/429 (228%) |
Catani | 2009 | NucliSens Easy Q HPV Assay BioMerieux | Automated | 16 18 31 33 45 | 81/180 (45%) |
Dockter* | 2009 | Aptima HPV Assay Tigris DST System | Semi-automated | 16 18 31 33 35 39 45 51 52 56 58 59 66 and 68 | 407/443 (919%) |
Dockter* | 2009 | Aptima HPV Assay DST | Automated | 16 18 31 33 35 39 45 51 52 56 58 59 66 and 68 | 410/443 (926%) |
Halfon | 2010 | NucliSens Easy Q HPV Assay BioMerieux | Automated | 16 18 31 33 45 | 61/89 (68.5%) |
Monsonego | 2010 | Aptima HPV Assay | Automated | 16 18 31 33 35 39 45 51 52 56 58 59 66 and 68 | 456/4429 (10.3%) |
Benevolo | 2011 | Pre-Tec HPV Proofer NASBA | Automated | 16 18 31 33 45 | 162/464 (36%) |
Ratnam | 2011 | Aptima HPV Assay | Automated | 16 18 31 33 35 39 45 51 52 56 58 59 66 and 68 | 964/1418 (68%) |
Our study | 2012 | Homebrew low-cost TRizol protocol | Manual | 16 18 31 45 | 36/60 (60%) |