Table 1 Primers designed for E6/E7 mRNA detection
HPV | Region | Size | Sequence |
|---|---|---|---|
16 | E6 | ± 659 pb | 5′ TAAAACTAAGGGCGTAACCG 3′ |
5′ TCTATTTCATCCTCCTCCTCTG 3′ | |||
16 | E7 | ± 491 pb | 5′ ACTGTGTCCTGAAGAAAAGCAA 3′ |
5′ AACCATCCATTACATCCCGT 3′ | |||
18 | E6 | ± 608 pb | 5′ TAGGTTGGGCAGCACATACT 3′ |
5′ ATACTTGTGTTTCTCTGCGTCG 3′ | |||
E7 | ± 358 pb | 5′ CGACGCAGAGAAACACAAGTAT 3′ | |
5′ ATTGTTGCTTACTGCTGGGAT 3′ | |||
31 | E6 | ± 606 pb | 5′ AAGTAGGGAGTGACCGAAAGT 3′ |
5′ ACAGTGGAGGTCAGTTGCC 3′ | |||
E7 | ± 437 pb | 5′ AGGCACGGCAAGAAAGACT 3′ | |
5′ AAAGAACCAGCCATTACACC 3′ | |||
45 | E6 | ± 659 pb | 5′ ATACTACATAAAAAAGGGTG 3′ |
5′ TCGTAACACAACAGGTCAACA 3′ | |||
E7 | ± 439 pb | 5′ AGGCACGGCAAGAAAGACT 3′ | |
5′ AAAGAACCAGCCATTACACC 3′ |