Skip to main content
SpringerPlus

Table 2 Primary antisera dilutions used in the immunohistochemical analysis

From: Immunohistochemical study of pituitary cells in wild and captive Salminus hilarii (Characiformes: Characidae) females during the annual reproductive cycle

Antiserum raised against

Code

Dilution

Chum salmon β-FSH

8510

1:1000

Chum salmon β-LH

8506

1:1000

Chum salmon PRL

8206

1:1000

Chum salmon SL

8906

1:2000

Chum salmon GH

8502

1:1000

Chum salmon β-FSH

2684

1:4000

Chum salmon β-LH

2686

1:4000

Mummichog β-FSH

299

1:1000

Mummichog β-LH

003

1:1000

Mummichog α-GtH

616

1:1000

Carp α-β-GtH

N/S

1:1000

Carp β-GtH

N/S

1:3000

  1. All the antisera used in this study were kindly provided by Drs: H. Kawauchi (School of Fisheries Sciences, Kitasato University, Iwate, Japan), A. Shimizu (National Research Institute of Fisheries Science, Fisheries Research Agency, Kanazawa, Yokohama, Japan) and J. Mancera (Facultad de Ciencias del Mar y Ambientales, Universidad de Cadiz, Spain).
  2. FSH, follicle-stimulating hormone; LH, luteinizing hormone; PRL, prolactin; SL, somatolactin; GH, growth hormone; N/S, not supplied.
Back to article page