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Figure 4

From: Immunohistochemical study of pituitary cells in wild and captive Salminus hilarii (Characiformes: Characidae) females during the annual reproductive cycle

Figure 4

Sagittal sections through the pituitary gland of Salminus hilarii . a-d ) Sections of pituitary showing the localization of α-β-GtHs and β-GtHs cells, using single immunocytochemistry, with the anti-carp antiserum (Dr. J. Mancera). a, b) Gonadotropins cells widely distributed in PPD; c, d) Details and differentiated GtH-ir cells (arrowhead) using anti-carp antisera, in a major magnitude of (a) and (b) respectively; e, f) Sections of the pituitary showing the localization of SL cells using single immunocytochemistry. Details of SL cells (arrowhead) (f) in high magnitfication of (e); g) periodic acid-Schiff (arrowheads indicate the PAS reactivity to SL cells); h) cross-reaction using SL antibody in GtH cells (arrowheads) in PPD region; i) PRL cells in RPD region with weakly cross-reaction, when using SL antibody. For more information, see the explanation in the text. RPD: rostral pars distalis; PPD: proximal pars distalis; PI: pars intermedia. Scale bars: a, b, e) 300 μm; c, d, i) 50 μm; f, g, h) 20 μm.

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