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Figure 2

From: Immunohistochemical study of pituitary cells in wild and captive Salminus hilarii (Characiformes: Characidae) females during the annual reproductive cycle

Figure 2

Sagittal sections through the pituitary gland of Salminus hilarii . a) Photomicrograph of the stained GH cells. Examples of measured nuclear area (red circle) and cell area (black circle) and the optical density (white square) were indicated; b-c) Sagittal sections of the pituitary stained using Mallory trichrome (b) and periodic acid-Schiff (PAS); d) Schematic sagittal representation of the pituitary, showing the distribution of ADH cells. RPD: rostral pars distalis; PPD: proximal pars distalis; PI: pars intermedia; NH: neurohypophysis; (■) prolactin cells; (●) growth hormone; (☆) gonadotropins cells; (▲) somatolactin cells; e) PRL-ir cells in the RPD. Details of PRL cells using the anti-chum salmon antisera (inset); f, g, h) GH-ir cells in the PPD (f) using the anti-chum salmon antisera. Details of cluster (arrowhead) of GH-ir cells (g) and isolated (arrowhead) of GH-ir cells (h). Scale bars: a, g-h) 20 μm; b-c, e-f ) 300 μm (20 μm insert).

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