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Figure 4 | SpringerPlus

Figure 4

From: Comparison of a quantitative real-time polymerase chain reaction (qPCR) with conventional PCR, bacterial culture and ELISA for detection of Mycobacterium avium subsp. paratuberculosis infection in sheep showing pathology of Johne’s disease

Figure 4

Standard curve for qPCR assay of MAP IS 900 gene quantification generated by plotting the known DNA concentrations of standard DNA template (3x10 7 copies) in serial ten-fold dilutions (10 -1 – 10 -6 ) against the corresponding threshold cycles (Ct values) of the amplification plots with MxPro TM QPCR software.

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