Figure 4From: Comparison of a quantitative real-time polymerase chain reaction (qPCR) with conventional PCR, bacterial culture and ELISA for detection of Mycobacterium avium subsp. paratuberculosis infection in sheep showing pathology of Johne’s disease Standard curve for qPCR assay of MAP IS 900 gene quantification generated by plotting the known DNA concentrations of standard DNA template (3x10 7 copies) in serial ten-fold dilutions (10 -1 – 10 -6 ) against the corresponding threshold cycles (Ct values) of the amplification plots with MxPro TM QPCR software. Back to article page