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Figure 7 | SpringerPlus

Figure 7

From: Contribution of extrahepatic small cells resembling small hepatocyte-like progenitor cells to liver mass maintenance in transplantation model of retrorsine-pretreated liver

Figure 7

Evaluation of ductular reaction after LT. (A), (B), (E)&(F) OV-6 immunohistochemistry staining. (C)&(D) H.E. staining. (A) Normal control without liver transplantation. Only cholangiocytes are positive for OV-6 staining. (B) Eight weeks after liver transplantation without retrorsine treatment (Group 1), oval cell reaction is not observed. (C) Retrorsine-pretreated liver four weeks after whole transplantation (Group 3). The liver section shows vigorous proliferation of bile ducts and small cells with oval nucleus (black arrow). Small hepatocyte-like cells (white arrow) are scarcely observed and formation of nodules is not clear in this field. (D) Retrorsine-pretreated liver eight weeks after partial liver transplantation (Group 4). Nodules of SHPCs (margined with white arrow) or relatively mature hepatocytes are large and abundant. The portal area is similar to Group 1 or 2, suggesting the completion of live regeneration. (E)&(F) Eight weeks after whole transplantation of retrorsine-pretreated liver (Group 3). The oval cell reaction persists at least until eight weeks when the whole liver with retrorsine-pretreatment is transplanted. Both intermediate hepatic cells (black arrow) and nodules of SHPCs are observed.

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