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Figure 4

From: Contribution of extrahepatic small cells resembling small hepatocyte-like progenitor cells to liver mass maintenance in transplantation model of retrorsine-pretreated liver

Figure 4

Clusters of GFP positive SHPCs-like cells in retrorsine-pretreated liver. Eight weeks after transplantation of retrorsine-pretreated liver, clusters of GFP positive cells are observed. These cells were not observed in Group 1 or 2. (A) Albumin staining. (B) GFP. (C) Merged image. (D) IHC staining of GFP for the same section. Nodule contains approximately 20 GFP positive cells in this plain. Surrounding hepatocytes are slightly larger than normal size hepatocytes. (E) Larger size nodule contains approximately 40 GFP positive cells. The nodule is beginning to compress the surrounding hepatocytes. Right upper part is nodule of GFP negative SHPCs. Note that the magnification is lower than D or F. (F) A cluster containing several sizes of GFP positive cells is also observed at lower frequency. A cluster of GFP positive SHPCs-like cells shows Ki67 positive staining. (G) Merged image of GFP (green) and Ki67 (red). (H) Higher magnification of same field with nucleus staining (blue). The Cluster of cells is surrounded by megalocytic hepatocytes with larger nucleus. Bar is 50 um.

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