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Figure 1

From: pPCV, a versatile vector for cloning PCR products

Figure 1

Construction of pPCV. (A) Restriction sites and predicted lacZα reading frame present in pPCV (B) Restriction analysis of pPCV. Lanes: M-2log molecular weight marker (New England Biolabs); 1-intact pPCV; 2-pPCV digested with Eco RV; 3-pPCV digested with Sac I and Kpn I; 4-pPCV digested with Xcm I. (C) Physical map of pPCV and its linearized forms pPCV-T and pPCV-B.

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