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Figure 5 | SpringerPlus

Figure 5

From: Hyperglycaemia and oxidative stress upregulate HSP60 & HSP70 expression in HeLa cells

Figure 5

Western blots of HSP60 expression in HeLa cells treated with heat shock, glucose, H2O2 and sodium azide over 3 and 7 days. a- Western blots of HSP60 and actin in cells treated with heat shock, glucose (100 mM), H2O2 (200 μM), and sodium azide (50 μM), respectively, over 3 days. The greatest induction of HSP60 is observed in the sodium azide treated sample, with similar levels of HSP60 observed in the glucose and hydrogen peroxide treatments. HSP60 was tested for a minimum of three times on different blots to provide an accurate representation of its induction. b- Western blots of HSP60 and actin in cells treated with heat shock, glucose (100 mM), H2O2 (200 μM), and sodium azide (50 μM) over 7 days. The greatest induction of HSP60 is observed in the sodium azide treated sample, followed by the hydrogen peroxide treatment, with samples treated with glucose having a lesser degree of induction. HSP60 was tested for a minimum of three times on different blots to provide an accurate representation of its induction.

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