Virulence, bacterocin genes and antibacterial susceptibility in Enterococcus faecalis strains isolated from water wells for human consumption

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Table 1 Oligonucleotide sequences used in this study and their products

Virulence genes	Oligonucleotide sequences	Product size (bp)	Reference
cylA	for5′-TGGATGATAGTGATAGGAAGT-3′	517	Eaton and Gasson 2001
	rev5′-TCTACAGTAAATCTTTCGTCA-3′
aggA	for5′-AAGAAAAAGTAGACCAAC-3′	1553	Eaton and Gasson 2001
	rev5′-AACGGCAAGACAAGTAAATA-3′
efaA	for5′-GACAGACCCTCACGAATA-3′	705	Eaton and Gasson 2001
	rev5′-AGTTCATCATGCTGCTGTAGTA-3′
eep	for5′-GAGCGGGTATTTTAGTTCGT-3′	937	Bittencourt de Marques and Suzart 2004
	rev5′-TACTCCAGCATTGGATGCT-3′
enlA	for5′-TTCTTCTTATTCTGTCAACGCAGC-3′	960	Bittencourt de Marques and Suzart 2004
	rev5′-GACTGTGAAATACCTATTTGCAAGC-3′
esp	for5′-TTGCTAATGCTAGTCCACGACC-3′	933	Shankar et al. 1999
	rev5′-GCGTCAACACTTGCATTGCCGAA-3′
Bacteriocin genes
as-48	for5′-GAGGAGGAGTATCATGGTTAAAG-3′	340	Sabia et al. 2008
	rev5′-CATATTGTTAAATTACCAAGCAATAA-3′
bac 31	for5′-GAAAAAGAAATTAGTTATTTGTG-3′	202	Sabia et al. 2008
	rev5′-CTATCTAGGAGCCCAAGG-3
entA	for5′-ATGAAACATTTAAAAATTTTGTCTA-3′	130	Sabia et al. 2008
	rev5′-TTAGCACTTCCCTGGAATTG-3′
entB	for5′-GAAAATGATCACAGAATGCCTA-3′	160	Du Toit et al. 2000
	rev5′-GTTGCATTTAGAGTATACATTTG-3′
entP	for5′-GTTAGTTTGTGACAAATTTTGG-3′	120	Sabia et al. 2008
	rev5′-GGTGTGGAAAAGCCGTTTC-3′
entL50 A/B	for5′-TGGGAGAATCGCAAAATTAG-3′	96	Du Toit et al. 2000
	rev5′-ATTGCCCATCCTTCTCCAAT-3′
ent1071	for5′-ATATTTAGGGGGACCGATAA-3′	405	Sabia et al. 2008
	rev5′-TATACATTCTTCCACTTATTTTT-3′