Figure 2

Immunocytochemistry of 293T cells using antibody IU-88. A, 293T cells were transfected with a plasmid encoding GFP-eIF5A, then immunostained using IU-88 and counterstained with DAPI to visualize nuclei; B, 293T cells were transfected with plasmids encoding GFP-eIF5A and DHS, then immunostained using IU-88 and counterstained with DAPI to visualize nuclei. In panels A and B, GFP is visualized in the green channel, IU-88 is visualized in the red channel, and DAPI is visualized in the blue channel. Magnification x100.