Figure 6
Transmission of RABV to mouse brainviaJAWS II cells harboring RABV. (A) Survival rates of five-week-old C57BL/6 mice injected intracerebrally with 106, 105, or 104 CVS- or ERA-infected JAWS II cells (n = 10 each group) at MOI of 30. Their survival was observed for 14 days after the transfer of the cells. The black and dotted lines represent the survival of the mice injected with CVS- and ERA-infected JAWS II cells, respectively. The circles indicate the survival of mice injected with 106 CVS- or ERA-infected JAWS II cells. The triangles indicate the survival of mice injected with 105 CVS- or ERA-infected JAWS II cells. The crosses indicate the survival of mice injected with 104 CVS- or ERA-inoculated JAWS II cells. The experiments were performed three times. The Kaplan-Meier method was used to analyze mouse survival. Statistical analyses were performed by log-rank test. (p < 0.01; 106 CVS: 106 ERA, 105 CVS: 105 ERA. p <0.05; 104 CVS: 104 ERA) (B) Half the mice from each group were sacrificed after seven days, and the presence of viral N mRNA and RABV N protein in the hippocampal tissues was determined by RT–PCR (mRNA) and a RABV N detection kit with FITC staining, respectively. The arrow indicates the positive band of N protein on the immunochromatographic test. The upper band corresponds to nonspecific control. The experiments were performed three times as shown in Figure 1B and representative pictures are shown.