Figure 5

Cell-to-cell infection of RABV from JAWS II cells to neural cells. A coculture assay of NA cells (indicator cells) with CVS-infected JAWS II cells or NA cells (donor cells) was performed as described in the Methods. The expression of the viral N protein was analyzed with a laser scanning microscope. After the infection of JAWS II cells with CVS at an MOI of 10, the cells were permeabilized with IntraPrep Permeabilization Reagent, and then reacted with FITC-conjugated anti-RABV N MAb. The experiments were performed three times and representative data are shown.