Figure 3

Expression of cell-surface molecules by flowcytometric analysis at 48 h after infection of CVS or ERA (MOI of 10). Profiles of the surface molecules on uninfected (control; white), CVS-infected (gray), and ERA-infected (black) JAWS II cells reacted with FITC conjugated anti-mouse MHC class I, MHC class II and CD80, PE conjugated anti-mouse CD86 and TLR3, and rat anti-mouse RIG1 antibodies, followed by a FITC-conjugated goat anti-rat IgG antibody. The relative fluorescence intensity of the surface molecules was determined with FACS. All cells including uninfected cells (control) were stained by the same procedure. The experiments were performed three times and error bars represent SEM. *p < 0.001, **p < 0.05, ***p < 0.01.