Figure 4From: Characterization of a new oxidant-stable serine protease isolated by functional metagenomics Estimation of the pH and temperature optima of SBcas3.3. a. Effect of pH on the relative activity of SBcas3.3. The ability to hydrolyse azocasein was assayed at 50°C in different buffers (♦) 50 mM Na-acetate buffer pH 5–6, (■) 50 mM Tris–HCl buffer pH 6–10, (▲) 50 mM glycine-NaOH buffer pH 8–12. b. Temperature-dependent activity of SBcas3.3, measured at pH 9 with azocasein as substrate. No error bars are indicated in these graphs (the standard error of the mean was always below 2%).Back to article page