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Figure 8 | SpringerPlus

Figure 8

From: DNA methylation-mediated silencing of PU.1 in leukemia cells resistant to cell differentiation

Figure 8

STAT1 tyrosine phosphorylation analysis of MEL DS19 and MEL-R cell lines. A) Non-stimulated MEL DS19 and MEL-R erythroleukemia cells (C) and stimulated with erythropoietin (100 U/ml) (Epo) or interferon α (IFN-α) (500 U/ml). Total cell lysates were immunoblotted with either anti-phospho-STAT1 (tyrosine 701) (upper panel) or anti-STAT1 antibody (lower panel). B) Comparison of Shp-1 expression by semi-quantitative RT-PCR of the parental MEL cells untreated (0) or treated with HMBA during 48 or 96 hours (48, 96) and of MEL-R cells (R). PCR products were normalized to GAPDH, separated by electrophoresis in a 1% agarose gel and stained with ethidium bromide.

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