Figure 4From: DNA methylation-mediated silencing of PU.1 in leukemia cells resistant to cell differentiation Methylation status of the PU.1 promoter region. A) DNA chromatogram of the PU.1 upstream region using bisulfite-treated genomic DNA derived from MEL and MEL-R cell lines. Red circles highlight the cytosines of the four CpG islands of the MEL-R cell line that changed to thymines in the MEL-DS19 cell line after bisulfite treatment. B) Methylation analysis of the PCR products of the bisulfite-treated genomic DNA from A). The CG sequences were numbered according to (Shearstone et al. 2011) and their methylation status was determined.Back to article page