Figure 3

Reactivation of the silenced PU.1 locus by 5-azaC treatment of MEL-R cells. Gene expression analysis of PU.1 using quantitative real time PCR (qRT-PCR) of MEL DS19 and MEL-R cell lines after treatment with 5-azaC. A) Non-induced MEL and MEL-R cells. MEL-R cells were routinely cultured in the presence of HMBA (+). B) MEL-R cells cultured for 24 hr in the absence (-) or presence (+) of 0.4 μM 5-azaC. C) MEL DS19 cells cultured during 24 hr in the absence (-) or presence (+) of 0.4 μM 5-azaC and 5 μM HMBA. The magnitude of gene expression was calculated using the ∆∆Ct method. Each value is the average of three readings.