Figure 2
From: DNA methylation-mediated silencing of PU.1 in leukemia cells resistant to cell differentiation
Map of the PU.1 genomic region showing the location and orientation of the SFFV proviral integration in MEL-DS19 cells. A) Diagram (drawn to scale) of the MEL-DS19 PU.1 genomic locus showing the location of a 3.4-kb HindIII (H) fragment referred to as the kb-14 URE (Upstream Regulatory Element) (Okuno et al. 2005), the SFFV genome is integrated downstream of the URE (gray box flanked by white boxes that symbolize the viral “long terminal repeats” (L)), and the five coding exons (black boxes) of the PU.1 locus. The sequences drawn below represent the upstream and downstream viral–host junctions, respectively. B) and C) Sequence alignment of the SFFV integration site displaying the PU.1 genomic sequences of mouse and of the MEL-DS19 cell line together with the SFFV genomic sequence. The PU.1 genomic sequence of the MEL-DS19 cell line corresponds to the upstream (B) and downstream (C) viral–host junction. Viral-host junctions as indicated in A) are underlined.