Figure 1From: DNA methylation-mediated silencing of PU.1 in leukemia cells resistant to cell differentiation PCR probe to confirm the SFFV integration site within the PU.1 locus. A) Illustration of the multiple verification PCR amplifications using MEL genomic DNA as a template. Black arrows over the SFFV genome (gray box) indicate the pair of primers designed to amplify the SFFV-PU.1 junctions. Odd numbers 1 and 3 represent the primers used to identify the upstream integration junction and even numbers 2 and 4 represent the primers used for the downstream integration. Number 5 represents the long-range PCR (LR-PCR) used as a probe to confirm the complete SFFV integration. Black boxes correspond to the five exons of PU.1; the arrow above exon number one represents the initiation and direction of translation. B) Agarose gel electrophoresis performed using the primers schematized in A ). C) Agarose gel electrophoresis of the LR-PCR probe to confirm SFFV integration; both the wild type (564 bp) and the integrated allele (6,859 bp) are visualized.Back to article page