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Figure 1 | SpringerPlus

Figure 1

From: DNA methylation-mediated silencing of PU.1 in leukemia cells resistant to cell differentiation

Figure 1

PCR probe to confirm the SFFV integration site within the PU.1 locus. A) Illustration of the multiple verification PCR amplifications using MEL genomic DNA as a template. Black arrows over the SFFV genome (gray box) indicate the pair of primers designed to amplify the SFFV-PU.1 junctions. Odd numbers 1 and 3 represent the primers used to identify the upstream integration junction and even numbers 2 and 4 represent the primers used for the downstream integration. Number 5 represents the long-range PCR (LR-PCR) used as a probe to confirm the complete SFFV integration. Black boxes correspond to the five exons of PU.1; the arrow above exon number one represents the initiation and direction of translation. B) Agarose gel electrophoresis performed using the primers schematized in A ). C) Agarose gel electrophoresis of the LR-PCR probe to confirm SFFV integration; both the wild type (564 bp) and the integrated allele (6,859 bp) are visualized.

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