Figure 2
From: HER-2 gene amplification in human breast cancer without concurrent HER-2 over-expression
A multiple-probe FISH analysis of the HER-2 and GRB7 segment from human chromosome 17q11-12. a A 140 kb genomic segment centered on HER-2 from human chromosome 17q11-12. a A 140 kb genomic segment centered on HER-2 from human chromosome 17q11-12. A. The stippled box represents the smallest amplified region from HER-2 previously identified. B. Five genes from this region shown are STARD3, PGAP3, HER-2, GRB7 and IKZF3. C. The mapping positions of two BACs (#3014 and #3079) that define the centromeric and telomeric ends of the smallest amplified region are shown. D. Filled box represents the extent of gene amplification from the HER-2 region for the cell line HCC-3153. b FISH analysis of human breast tumor cells from tumor #031. HER-2 FISH was performed using the Abbott PathVysion kit on 4 μm frozen sections. Probe for the HER-2 locus (17q12, SpectrumOrange) and the chromosome 17 centromere (CEP17) as the chromosome 17 control probe (D17Z1,17p11.1-q11.1, SpectrumGreen). BAC 3079, BAC 3014. Purified 5′probe #3014 was labeled with Abbott SpectrumGreen (green fluorescence). Purified 3′probe #3079 was labeled with Abbott SpectrumOrange (orange fluorescence), and the commercially available chromosome 17 centromere probe with Abbott SpectrumAqua (aqua fluorescence). Coalescence of orange and green fluorescence signals yield yellow signals indicating that sequences represented by both probes are amplified in one contiguous unit.