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Figure 2 | SpringerPlus

Figure 2

From: Bias problems in culture-independent analysis of environmental bacterial communities: a representative study on hydrocarbonoclastic bacteria

Figure 2

DGGE of 16S rDNA amplicons in total DNA extracts from six pristine (P) and nearby six oil-polluted (O) seawater samples collected from the Arabian Gulf coast of Kuwait. (a) DGGE gel; in contrast to the cases of the “closed” desert samples (Figure 3), the DGGE bands of the “open” seawater samples were similar as far as the band numbers and patterns are concerned. The total numbers of the bands for all samples were not much higher than the total number of species recorded in each sample using culture-based analysis (Al-Awadhi et al. 2012). DNAs in individual bands were amplified, sequenced and the sequences were compared with those (cultured and uncultured) of the closest species in the GenBank (results in Table 1, which presents also information related to the sequencing). (b) Cluster analysis of DGGE-results using Euclidean distances.

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