Table 4 Name, BAC-End sequence name, motif, reference, forward and reverse primer and annealing temperature of the SSR markers used
Name | BAC-End sequence name | Motif | Reference | Forward Primer | Reverse Primer | TºC |
|---|---|---|---|---|---|---|
SP 1 | Pbac -102C-06.T1.G06 | (TTTC)5/(TTC)9 | Chun et al., (2006) | TGCAACAGAAATAAAAACAGCA | GACGTGGACGAGCTCTGTGT | 51 |
SP 3 | Pbac -14D-B01.t1_009.ab1 | (AC)9 | Chun et al., (2006) | CACCAACAAGTTCCTTGGGT | TGCATGCATGTGTGTGGATA | 57 |
SP4 | Pbac -15C-B06.t1.B06.ab1 | (AT)9 | Chun et al., (2006) | TGCTCATAAAGTGATGGAGGT | TGGCGACCATTTAAACAACA | 55.5 |
SP5 | Pbac -15C-E11.y1.E11.ab1 | (AC)9 | Chun et al., (2006) | TTGGCTTCAAATTCAGGCTT | GCGGCTTCTGGATCTGATAA | 56 |
SP6 | Pbac -16C-C10.y1.C10.ab1 | (AT)9 | Chun et al., (2006) | CTTGCACCGAACCCTAAAAG | CATGAAAAACACATGCCTGC | 57 |
SP7 | Pbac -16A-B08.y1_063.ab1 | (AAT)7 | Chun et al., (2006) | CAGTTGTAGGGGTTGGTGGT | GTCCACAAATCAGAGCCCAT | 59 |
SP8 | Pbac -2B-F07.Y1_061 | (ATT)7 | Chun et al., (2006) | CAAATCATGTTGGTCTGCGT | GCTCAGCGGCTATTTTTGAC | 57 |
SP9 | Pbac -28D-E10.t1_081.ab1 | (AC)10 | Chun et al., (2006) | TCAATGAGCCCCTCAATTTC | ATGGATGGATTCAGCCGTTA | 56 |
SP10 | Pbac -3C-H06.t1_057.ab1 | (CT)20 | Chun et al., (2006) | CGACGTCGTTTTCTCCTTTC | CACACATCGTGGGTTGAAGT | 58 |
SSPA 1 | Pbac-102A-H04.Y1.h04.scf | (A)21 | Eustice et al., (2008) | TCATCGTCTTCAACCTGTAGC | ATCGACCTCCTCCATCACAC | 61 |
SSPA 2 | Pbac-102C-01.T1.H01.scf | (AT)12 | Eustice et al., (2008) | ACCAGAGTGGACCCAGTAGC | TGTTCACGTAAGGCATCCTG | 61 |
SSPA 3 | Pbac-102D-A11.T1.A11.scf | (AG)10 | Eustice et al., (2008) | CGAAGCAAAACTTCTCAGCC | TCTCAATTTCCATTTTCCGC | 58 |
SSPA 4 | Pbac-10B-F10.T1_089.ab1 | (TTC)10 | Eustice et al., (2008) | GTGCAAGTCTCTCGAGTCCC | CTTGCTTTGCACTTTTCAGG | 61 |
SSPA 5 | Pbac-10C-c04.y1_023.ab1 | (TA)11 | Eustice et al., (2008) | CACGAACAACTGTCACCCAC | TCAAGACCTTTGCATGATGG | 61 |
SSPA 6 | Pbac-10C-G06.t1_049.ab1 | (TA)12 | Eustice et al., (2008) | GCTGCATCGACATTTACGAA | TCAAGCCTGAGGAATCTGCT | 59 |
SSPA 7 | Pbac-1D-F03.y1_028.ab1 | (AG)24 | Eustice et al., (2008) | TTCAAATCTTTTTCGCACCC | TCAACAGCTTCGTTGACCAG | 59 |
SSPA 8 | Pbac-21C-F01.t1F01.scf | (AT)12 | Eustice et al., (2008) | TGTCTCAGCATATCCACCCA | ATGGCCTTTTGGAACATCAG | 60 |
SSPA 9 | Pbac-25ZB-F01.y1_012.ab1 | (AAG)10 | Eustice et al., (2008) | GCAGAAGCCAACAGCTCTCT | AGATCTAGCAGCCGCCATAA | 61 |
SSPA 10 | Pbac-28A-B03.t1_026.ab1 | (TTC)7 | Eustice et al., (2008) | AGGAATGCCCTCCATGTAAA | AGGAATGCCCTCCATGTAAA | 59 |
SP 3 | Pbac -14D-B01.t1_009.ab1 | (AC)9 | Chun et al., (2006) | CACCAACAAGTTCCTTGGGT | TGCATGCATGTGTGTGGATA | 57 |