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Table 1 Experimental condition of in-vitro tests

From: Use of in-vitro experimental results to model in-situ experiments: bio-denitrification under geological disposal conditions

Series

Run

Method

Initial Condition*1

Microorganism

Electron Donor

Duration

pH

Conc. of NO3

Activated Sludge

Azoarcus Tolulyticus

Glucose

Super Plasticizer

Mixture of HA*2

Sodium Salt of HA*3

 

(mmol/dm3)

(mg/dm3)

(mg/dm3)

(mg/dm3)

(mg/dm3)

(mg/dm3)

(mg/dm3)

(d)

A

1

Ampule

7

20

1000

-

-

0

 

-

50

2

Ampule

7

20

1000

-

-

3000

 

-

6–50

B

3

Ampule

8

10

1000

-

-

-

100

-

10

4

Ampule

8

50

1000

-

-

-

100

-

10

5

Vial

8

10

1000

-

-

-

1

-

0–30

6

Vial

8

10

1000

-

-

-

10

-

0–30

7

Vial

8

10

1000

-

-

-

100

-

0–30

C

8

Ampule

8

50

-

1000

-

-

-

0

11

9

Ampule

8

50

-

1000

-

-

-

10

11

10

Ampule

8

50

-

1000

-

-

-

100

11

11

Ampule

8

50

-

1000

-

-

-

1000

11

D

12

Vial

8

50

-

1000

100

-

-

-

0.2–7

13

Vial

8

50

-

1000

-

-

-

100

0.2–7

E

14

Vial

8

50

10

-

-

-

-

0

1–50

15

Vial

8

50

10

-

-

-

-

10

1–50

  1. *1 In every experiments, temperature was controlled at 35 degree-Celcius and sealed gas was Argon. *2 Humic acids (HA) from Japan Humic Substance Association, *3 Reagent grade humic acids from Aldrich.