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Table 1 Experimental condition of in-vitro tests

From: Use of in-vitro experimental results to model in-situ experiments: bio-denitrification under geological disposal conditions

Series Run Method Initial Condition*1 Microorganism Electron Donor Duration
pH Conc. of NO3 Activated Sludge Azoarcus Tolulyticus Glucose Super Plasticizer Mixture of HA*2 Sodium Salt of HA*3
  (mmol/dm3) (mg/dm3) (mg/dm3) (mg/dm3) (mg/dm3) (mg/dm3) (mg/dm3) (d)
A 1 Ampule 7 20 1000 - - 0   - 50
2 Ampule 7 20 1000 - - 3000   - 6–50
B 3 Ampule 8 10 1000 - - - 100 - 10
4 Ampule 8 50 1000 - - - 100 - 10
5 Vial 8 10 1000 - - - 1 - 0–30
6 Vial 8 10 1000 - - - 10 - 0–30
7 Vial 8 10 1000 - - - 100 - 0–30
C 8 Ampule 8 50 - 1000 - - - 0 11
9 Ampule 8 50 - 1000 - - - 10 11
10 Ampule 8 50 - 1000 - - - 100 11
11 Ampule 8 50 - 1000 - - - 1000 11
D 12 Vial 8 50 - 1000 100 - - - 0.2–7
13 Vial 8 50 - 1000 - - - 100 0.2–7
E 14 Vial 8 50 10 - - - - 0 1–50
15 Vial 8 50 10 - - - - 10 1–50
  1. *1 In every experiments, temperature was controlled at 35 degree-Celcius and sealed gas was Argon. *2 Humic acids (HA) from Japan Humic Substance Association, *3 Reagent grade humic acids from Aldrich.