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Table 4 Properties of various samples from fractionation by ultrafiltration

From: Optimization of the production and characterization of milk clotting enzymes by Bacillus subtilis natto

Activity MCA (SU/mL) Protein Con. (mg/mL) Proteolytic activity (OD660) MCA/PA Ratiio
Fraction     
Initial Broth 960 ± 9 0.188 ± 0.006 0.115 ± 0.003 8348 ± 231
Concentrated Retentate 3200 ± 80a (480 ± 12)b 0.231 ± 0.005 a (0.174 ± 0.008)b 0.108 ± 0.004 a (0.104 ± 0.003)b 29630 ± 1323a (4615 ± 176)b
Filtrate 228 ± 11a (1200 ± 25)b 0.116 ± 0.010a (0.224 ± 0.009)b 0.089 ± 0.004a (0.111 ± 0.005)b 2561 ± 168a (10811 ± 535)b
  1. aValues calculated for samples obtained using membrane having MWCO 5 kDa.
  2. bValues (in the parenthesis) calculated for samples obtained using membrane having MWCO 50 kDa.