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Table 4 Properties of various samples from fractionation by ultrafiltration

From: Optimization of the production and characterization of milk clotting enzymes by Bacillus subtilis natto

Activity

MCA (SU/mL)

Protein Con. (mg/mL)

Proteolytic activity (OD660)

MCA/PA Ratiio

Fraction

    

Initial Broth

960 ± 9

0.188 ± 0.006

0.115 ± 0.003

8348 ± 231

Concentrated Retentate

3200 ± 80a (480 ± 12)b

0.231 ± 0.005 a (0.174 ± 0.008)b

0.108 ± 0.004 a (0.104 ± 0.003)b

29630 ± 1323a (4615 ± 176)b

Filtrate

228 ± 11a (1200 ± 25)b

0.116 ± 0.010a (0.224 ± 0.009)b

0.089 ± 0.004a (0.111 ± 0.005)b

2561 ± 168a (10811 ± 535)b

  1. aValues calculated for samples obtained using membrane having MWCO 5 kDa.
  2. bValues (in the parenthesis) calculated for samples obtained using membrane having MWCO 50 kDa.