Table 2 Experimental design and results of the central composite design together with predicted yields from the model equation
Factorsa | X1 (g/L) | X2 (g/L) | X3 (g/L) | X4 (g/L) | MCA (SU/mL) | Predicted Values |
|---|---|---|---|---|---|---|
Trial | ||||||
1 | 20 | 3 | 1.5 | 8 | 40.00 | 99.639 |
2 | 40 | 3 | 1.5 | 8 | 600.00 | 600.917 |
3 | 20 | 7 | 1.5 | 8 | 20.00 | 85.639 |
4 | 40 | 7 | 1.5 | 8 | 533.33 | 534.747 |
5 | 20 | 3 | 2.5 | 8 | 133.33 | 231.47 |
6 | 40 | 3 | 2.5 | 8 | 800.00 | 783.084 |
7 | 20 | 7 | 2.5 | 8 | 80.00 | 120.305 |
8 | 40 | 7 | 2.5 | 8 | 600.00 | 619.749 |
9 | 20 | 3 | 1.5 | 16 | 233.33 | 297.304 |
10 | 40 | 3 | 1.5 | 16 | 800.00 | 826.417 |
11 | 20 | 7 | 1.5 | 16 | 25.00 | 108.639 |
12 | 40 | 7 | 1.5 | 16 | 600.00 | 585.582 |
13 | 20 | 3 | 2.5 | 16 | 333.33 | 398.635 |
14 | 40 | 3 | 2.5 | 16 | 960.00 | 978.084 |
15 | 20 | 7 | 2.5 | 16 | 30.00 | 112.805 |
16 | 40 | 7 | 2.5 | 16 | 633.33 | 640.084 |
17 | 10 | 5 | 2 | 12 | 30.00 | −174.501 |
18 | 50 | 5 | 2 | 12 | 800.00 | 854.056 |
19 | 30 | 1 | 2 | 12 | 700.00 | 617.442 |
20 | 30 | 9 | 2 | 12 | 333.33 | 265.442 |
21 | 30 | 5 | 1 | 12 | 450.00 | 381.611 |
22 | 30 | 5 | 3 | 12 | 650.00 | 567.944 |
23 | 30 | 5 | 2 | 4 | 450.00 | 390.777 |
24 | 30 | 5 | 2 | 20 | 700.00 | 608.777 |
b25(C) | 30 | 5 | 2 | 12 | 533.33 | 542.167 |
b26(C) | 30 | 5 | 2 | 12 | 571.43 | 542.167 |
b27(C) | 30 | 5 | 2 | 12 | 521.74 | 542.167 |