Skip to main content

Advertisement

Table 2 Experimental design and results of the central composite design together with predicted yields from the model equation

From: Optimization of the production and characterization of milk clotting enzymes by Bacillus subtilis natto

Factorsa X1 (g/L) X2 (g/L) X3 (g/L) X4 (g/L) MCA (SU/mL) Predicted Values
Trial       
1 20 3 1.5 8 40.00 99.639
2 40 3 1.5 8 600.00 600.917
3 20 7 1.5 8 20.00 85.639
4 40 7 1.5 8 533.33 534.747
5 20 3 2.5 8 133.33 231.47
6 40 3 2.5 8 800.00 783.084
7 20 7 2.5 8 80.00 120.305
8 40 7 2.5 8 600.00 619.749
9 20 3 1.5 16 233.33 297.304
10 40 3 1.5 16 800.00 826.417
11 20 7 1.5 16 25.00 108.639
12 40 7 1.5 16 600.00 585.582
13 20 3 2.5 16 333.33 398.635
14 40 3 2.5 16 960.00 978.084
15 20 7 2.5 16 30.00 112.805
16 40 7 2.5 16 633.33 640.084
17 10 5 2 12 30.00 −174.501
18 50 5 2 12 800.00 854.056
19 30 1 2 12 700.00 617.442
20 30 9 2 12 333.33 265.442
21 30 5 1 12 450.00 381.611
22 30 5 3 12 650.00 567.944
23 30 5 2 4 450.00 390.777
24 30 5 2 20 700.00 608.777
b25(C) 30 5 2 12 533.33 542.167
b26(C) 30 5 2 12 571.43 542.167
b27(C) 30 5 2 12 521.74 542.167
  1. aStarch (X1), Corn steep powder (X2), Soybean meal (X3) and Dry milk (X4) ; bCentrepoints.