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Table 2 Biological activity of FVIII secreted by Hek-293/BMN-FVIIIΔB-I-GFP

From: Quantitative correlation between transcriptional levels of ER chaperone, peroximal protein and FVIII productivity in human Hek-293 cell line

Sample % of GFP expression Activity (%) IU/mL
Culture medium DMEN - 1.5 -
S. Cell pop. 01 74.0 14.0 0.1
S. Cell pop. 02 80.0 11.1 0.1
S. Cell pop. 03 76.5 10.0 0.1
S. Cell pop. 04 72.1 34.6 0.3
S. Cell pop. 05 70.0 41.9 0.4
S. Cell pop. 06 70.5 21.7 0.2
S. Cell pop. 07 68.1 1.9 0.0
S. Cell pop. 08 63.8 2.2 0.0
S. Cell pop. 09 44.3 2.9 0.0
S. Cell pop. 10 43.4 20.1 0.2
S. Cell pop. 11 44.9 4.1 0.0
S. Cell pop. 12 48.2 75.8 0.7
S. Cell pop. 13 53.6 18.5 0.1
S. Cell pop. 14 62.5 47.9 0.4
S. Cell pop. 15 48.6 37.1 0.3
  1. Analysis of biological activity of FVIII secreted by Hek-293/BMN-FVIIIΔB-I-GFP using TTPA assay. S Supernatant, Cell pop Cell population, Hek-293 human embryonic kidney epithelial cells.