|
Sample
|
% of GFP expression
|
Activity (%)
|
IU/mL
|
|---|
|
Culture medium DMEN
|
-
|
1.5
|
-
|
|
S. Cell pop. 01
|
74.0
|
14.0
|
0.1
|
|
S. Cell pop. 02
|
80.0
|
11.1
|
0.1
|
|
S. Cell pop. 03
|
76.5
|
10.0
|
0.1
|
|
S. Cell pop. 04
|
72.1
|
34.6
|
0.3
|
|
S. Cell pop. 05
|
70.0
|
41.9
|
0.4
|
|
S. Cell pop. 06
|
70.5
|
21.7
|
0.2
|
|
S. Cell pop. 07
|
68.1
|
1.9
|
0.0
|
|
S. Cell pop. 08
|
63.8
|
2.2
|
0.0
|
|
S. Cell pop. 09
|
44.3
|
2.9
|
0.0
|
|
S. Cell pop. 10
|
43.4
|
20.1
|
0.2
|
|
S. Cell pop. 11
|
44.9
|
4.1
|
0.0
|
|
S. Cell pop. 12
|
48.2
|
75.8
|
0.7
|
|
S. Cell pop. 13
|
53.6
|
18.5
|
0.1
|
|
S. Cell pop. 14
|
62.5
|
47.9
|
0.4
|
|
S. Cell pop. 15
|
48.6
|
37.1
|
0.3
|
- Analysis of biological activity of FVIII secreted by Hek-293/BMN-FVIIIΔB-I-GFP using TTPA assay. S Supernatant, Cell pop Cell population, Hek-293 human embryonic kidney epithelial cells.
