Cloning FVIIIΔB in pBMN-I-GFP. FVIII∆B containing FVIII heavy and light chain with B-domain partial deleted was cloned first in pCR2.1-TOPO plasmid and after in pBMN-I-GFP. Agarose gel (1%) stained with ethidium bromide after electrophoresis showing both clones. Lanes 1 and 3: DNA from recombinant clones without restriction enzyme digestion. Lane 2: Xho I/Not I digested positive FVIII∆B clone in pCR2.1-TOPO plasmid. Lane 4: Xho I/Not I digested positive FVIII∆B clone in pBMN-I-GFP plasmid. Lanes M: 1 kb DNA ladder and lambda DNA digested with Hind III.