Table 4 Comparative performance for production of some biopharmaceutical proteins by E . coli BL21(DE3) fed-batch cultures in complex medium

Human soluble B lymphocyte stimulator (hsBLyS) 17-18 kDa

Glucose/lactose

34

0.92

0.11

0.120

3.70

Li et al. 2006

Tilapia insulin-like growth factor 2 - 7 kDa

Glucose/IPTG

30

5.20

0.32

0.062

9.69

Hu et al. 2004

Streptokinase 47 kDa

Glucose/IPTG

11

1.31

0.10

0.050

1.12

Goyal et al. 2009

Anti-cancer drug TATm-survivin (T34A) 17 kDa

Glucose/IPTG

14

0.63

0.12

0.191

1.68

Zhang et al. 2009 (t = 14 h)

Anti-HIV protein Griffthsin (GRFT) 14.5 kDa

Auto induction (Glucose + glycerol + lactose)/lactose

15

0.89^a

0.05

0.061^a

0.82

Giomarelli et al. 2006

this work:

SpaA from E. rhusiopathiae 42 kDa

Glucose/IPTG

11.5

1.82

0.25

0.136

2.85

Exp #1 (t = 11.5 h)

Auto induction (Glucose + glycerol + lactose)/lactose

20

1.16

0.18

0.158

1.47

Exp #2 (t = 8 h)

Glicerol/lactose

20

2.18

0.32

0.155

5.97

Exp #3 (t = 20 h)

Glycerol + glucose/lactose

16

4.13

0.39

0.095

6.35

Exp #4 (t = 16 h)