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Table 4 Comparative performance for production of some biopharmaceutical proteins by E . coli BL21(DE3) fed-batch cultures in complex medium

From: Non-conventional induction strategies for production of subunit swine erysipelas vaccine antigen in rE. coli fed-batch cultures

Product & molecular weight Carbon source/inducer Culture time (h) Biomass productivity (gDCW.L-1.h-1) Protein volumetric productivity (gprot.L-1.h-1) Specific protein production (gprot.gDCW -1) Product content (g.L-1) Reference
Human soluble B lymphocyte stimulator (hsBLyS) 17-18 kDa Glucose/lactose 34 0.92 0.11 0.120 3.70 Li et al. 2006
Tilapia insulin-like growth factor 2 - 7 kDa Glucose/IPTG 30 5.20 0.32 0.062 9.69 Hu et al. 2004
Streptokinase 47 kDa Glucose/IPTG 11 1.31 0.10 0.050 1.12 Goyal et al. 2009
Anti-cancer drug TATm-survivin (T34A) 17 kDa Glucose/IPTG 14 0.63 0.12 0.191 1.68 Zhang et al. 2009 (t = 14 h)
Anti-HIV protein Griffthsin (GRFT) 14.5 kDa Auto induction (Glucose + glycerol + lactose)/lactose 15 0.89a 0.05 0.061a 0.82 Giomarelli et al. 2006
this work:        
SpaA from E. rhusiopathiae 42 kDa Glucose/IPTG 11.5 1.82 0.25 0.136 2.85 Exp #1 (t = 11.5 h)
  Auto induction (Glucose + glycerol + lactose)/lactose 20 1.16 0.18 0.158 1.47 Exp #2 (t = 8 h)
  Glicerol/lactose 20 2.18 0.32 0.155 5.97 Exp #3 (t = 20 h)
  Glycerol + glucose/lactose 16 4.13 0.39 0.095 6.35 Exp #4 (t = 16 h)
  1. aestimated values, assuming 1 unit of Optical Density at 600 nm (OD600nm = 1.0) equivalent to 0.5 gDCW.
  2. “t” corresponds to the cultivation time where the maximum values for specific protein production were reached (for each experiment).