Non-conventional induction strategies for production of subunit swine erysipelas vaccine antigen in rE. coli fed-batch cultures

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Table 4 Comparative performance for production of some biopharmaceutical proteins by E . coli BL21(DE3) fed-batch cultures in complex medium

Product & molecular weight	Carbon source/inducer	Culture time (h)	Biomass productivity (g_DCW.L^-1.h^-1)	Protein volumetric productivity (g_prot.L^-1.h^-1)	Specific protein production (g_prot.g_DCW ^-1)	Product content (g.L^-1)	Reference
Human soluble B lymphocyte stimulator (hsBLyS) 17-18 kDa	Glucose/lactose	34	0.92	0.11	0.120	3.70	Li et al. 2006
Tilapia insulin-like growth factor 2 - 7 kDa	Glucose/IPTG	30	5.20	0.32	0.062	9.69	Hu et al. 2004
Streptokinase 47 kDa	Glucose/IPTG	11	1.31	0.10	0.050	1.12	Goyal et al. 2009
Anti-cancer drug TATm-survivin (T34A) 17 kDa	Glucose/IPTG	14	0.63	0.12	0.191	1.68	Zhang et al. 2009 (t = 14 h)
Anti-HIV protein Griffthsin (GRFT) 14.5 kDa	Auto induction (Glucose + glycerol + lactose)/lactose	15	0.89^a	0.05	0.061^a	0.82	Giomarelli et al. 2006
this work:
SpaA from E. rhusiopathiae 42 kDa	Glucose/IPTG	11.5	1.82	0.25	0.136	2.85	Exp #1 (t = 11.5 h)
	Auto induction (Glucose + glycerol + lactose)/lactose	20	1.16	0.18	0.158	1.47	Exp #2 (t = 8 h)
	Glicerol/lactose	20	2.18	0.32	0.155	5.97	Exp #3 (t = 20 h)
	Glycerol + glucose/lactose	16	4.13	0.39	0.095	6.35	Exp #4 (t = 16 h)

^aestimated values, assuming 1 unit of Optical Density at 600 nm (OD_600nm = 1.0) equivalent to 0.5 g_DCW.
“t” corresponds to the cultivation time where the maximum values for specific protein production were reached (for each experiment).