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Table 1 Media composition used for bioreactor cultivations

From: Non-conventional induction strategies for production of subunit swine erysipelas vaccine antigen in rE. coli fed-batch cultures

  Experiment #1 Experiment #2 Experiment #3 Experiment #4
Nutrient (g.L-1) Batch Fed-batch Batch Fed-batch Batch Fed-batch Batch Fed-batch Induction
Glucose 10 240.0 8.0 --- --- --- 10.0 --- ---
Glycerol --- --- 10.0 400.0 40.0 400.0 60.0 400.0 ---
Lactose --- --- 2.0 160.0 --- 80.0 --- --- 20a
Tryptone 10.0 10.0 10.0 10.0 10.0 10.0 10.0 10.0 10.0
Yeast extract 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0
NaCl 5.0 5.0 --- --- --- --- --- --- ---
MgSO4.7H2O 2.5 20.0 0.5 40.0 0.5 40.0 0.5 40.0 0.5
K2HPO4 7.0 7.0 --- --- --- --- --- --- ---
KH2PO4 --- --- 3.4 3.4 3.4 3.4 3.4 3.4 3.4
Na2HPO4.12H2O --- --- 9.0 9.0 9.0 9.0 9.0 9.0 9.0
NH4Cl --- --- 2.7 2.7 2.7 2.7 2.7 2.7 2.7
Na2SO4 --- --- 0.7 0.7 0.7 0.7 0.7 0.7 0.7
kanamycin (μg.mL-1) 100 100 100 100 100 100 100 100 100
Antifoam PPG 30% (mL.L-1) 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0
Metal solution no no yes yes yes yes yes yes yes
  1. afinal concentration in the bioreactor.