Table 1 Media composition used for bioreactor cultivations
Experiment #1 | Experiment #2 | Experiment #3 | Experiment #4 | ||||||
|---|---|---|---|---|---|---|---|---|---|
Nutrient (g.L-1) | Batch | Fed-batch | Batch | Fed-batch | Batch | Fed-batch | Batch | Fed-batch | Induction |
Glucose | 10 | 240.0 | 8.0 | --- | --- | --- | 10.0 | --- | --- |
Glycerol | --- | --- | 10.0 | 400.0 | 40.0 | 400.0 | 60.0 | 400.0 | --- |
Lactose | --- | --- | 2.0 | 160.0 | --- | 80.0 | --- | --- | 20a |
Tryptone | 10.0 | 10.0 | 10.0 | 10.0 | 10.0 | 10.0 | 10.0 | 10.0 | 10.0 |
Yeast extract | 5.0 | 5.0 | 5.0 | 5.0 | 5.0 | 5.0 | 5.0 | 5.0 | 5.0 |
NaCl | 5.0 | 5.0 | --- | --- | --- | --- | --- | --- | --- |
MgSO4.7H2O | 2.5 | 20.0 | 0.5 | 40.0 | 0.5 | 40.0 | 0.5 | 40.0 | 0.5 |
K2HPO4 | 7.0 | 7.0 | --- | --- | --- | --- | --- | --- | --- |
KH2PO4 | --- | --- | 3.4 | 3.4 | 3.4 | 3.4 | 3.4 | 3.4 | 3.4 |
Na2HPO4.12H2O | --- | --- | 9.0 | 9.0 | 9.0 | 9.0 | 9.0 | 9.0 | 9.0 |
NH4Cl | --- | --- | 2.7 | 2.7 | 2.7 | 2.7 | 2.7 | 2.7 | 2.7 |
Na2SO4 | --- | --- | 0.7 | 0.7 | 0.7 | 0.7 | 0.7 | 0.7 | 0.7 |
kanamycin (μg.mL-1) | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 |
Antifoam PPG 30% (mL.L-1) | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 |
Metal solution | no | no | yes | yes | yes | yes | yes | yes | yes |