Figure 6
SDS-PAGE of cell lysates from samples collected during bioreactor cultures of E . coli BL21(DE3) expressing rSpaA under different culture and induction conditions (Exp #1 to #4). Lane 1: Exp #1 (t = 11.5 h); Lane 2: Exp #2 (t = 8 h); Lane 3: Exp #3 (t = 20 h); Lane 4: Exp #4 (t = 16 h); Lane 5: Molecular mass markers (kDa). Samples were collected at the cultivation time (t) indicated in parentheses, that corresponds to the moment of maximum specific protein production for each experiment.