Figure 2From: Proliferative effect and osteoinductive potential of extracellular matrix coated on cell culture plates Preparation and VG histochemical staining of osteoblast-ECM and fibroblast-ECM. Osteoblasts and fibroblasts were removed after treatment with PBS containing 0.5% Triton X-100 and 0.10 M NH4OH, and the ECMs formed on the surfaces were revealed. VG staining with VG kit containing hematoxylin solution showed collagen fibers which were stained red and no nuclei. (bar: 100 μm).Back to article page