Acinetobacter baumannii producing OXA-23 detected in the Czech Republic

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Table 1 Primers for detecting carbapenemases of class A and two selected class D subgroups

Primer	Sequence	Product size (bp)	T_a	Reference
Primer	(5′→3′)	Product size (bp)	T_a	Reference
SME-F	AGATAGTAAATTTTATAG	1138	50°C	Radice et al.; 2004
SME-R	CTCTAACGCTAATAG	1138	50°C	Radice et al.; 2004
IMI-F	ATAGCCATCCTTGTTTAGCTC	818	50°C	Queenan et al.; 2000
IMI-R	TCTGCGATTACTTTATCCTC	818	50°C	Queenan et al.; 2000
NMC1	GCATTGATATACCTTTAGCAGAGA	2158	50°C	Aubron et al.; 2005
NMC4	CGGTGATAAAATCACACTGAGCATA	2158	50°C	Aubron et al.; 2005
KPC-F	ATGTCACTGTATCGCCGTCT	893	60°C	Bradford et al.; 2004
KPC-R	TTTTCAGAGCCTTACTGCCC	893	60°C	Bradford et al.; 2004
GES-F	GTTTTGCAATGTGCTCAACG	371	50°C	Weldhagen et al.; 2004
GES-R	TGCCATAGCAATAGGCGTAG	371	50°C	Weldhagen et al.; 2004
OXA-23 F	AAGCATGATGAGCGCAAAG	1066	50°C	Donald et al.; 2000
OXA-23R	AAAAGGCCCATTTATCTCAAA	1066	50°C	Donald et al.; 2000
OXA-48 F	TTGGTGGCATCGATTATCGG	744	50°C	Poirel et al., 2004
OXA-48R	GAGCACTTCTTTTGTGATGGC	744	50°C	Poirel et al., 2004