Table 3 Central composite design matrix for the experimental and predicted results in the production of nitrilase
From: Enhancing nitrilase production from Fusarium proliferatum using response surface methodology
Run | Glucose (g/l) | Sodium nitrate (g/l) | ϵ-caprolactam (g/l) | Predicted activity(U/g) | Actual activity(U/g) |
|---|---|---|---|---|---|
1 | 50.00 | 1.66 | 3.50 | 48.65 | 48 ±2.06 |
2 | 50.00 | 2.50 | 1.82 | 32.03 | 30 ±2.24 |
3 | 66.82 | 2.50 | 3.50 | 54.20 | 50 ±1.35 |
4 | 50.00 | 2.50 | 3.50 | 58.14 | 59 ±1.24 |
5 | 33.18 | 2.50 | 3.50 | 51.57 | 49 ±2.35 |
6 | 40.00 | 3.00 | 4.50 | 42.88 | 44 ±1.32 |
7 | 40.00 | 3.00 | 2.50 | 44.77 | 45 ±1.23 |
8 | 50.00 | 3.34 | 3.50 | 46.12 | 46 ±1.42 |
9 | 60.00 | 3.00 | 2.50 | 44.33 | 45 ±1.34 |
10 | 50.00 | 2.50 | 3.50 | 58.14 | 56 ±2.33 |
11 | 40.00 | 2.00 | 2.50 | 38.27 | 41 ±2.31 |
12 | 50.00 | 2.50 | 5.18 | 34.74 | 30 ±3.24 |
13 | 60.00 | 2.00 | 2.50 | 44.33 | 48 ±2.52 |
14 | 50.00 | 2.50 | 3.50 | 58.14 | 55 ±3.26 |
15 | 40.00 | 2.00 | 4.50 | 45.88 | 50 ±3.94 |
16 | 50.00 | 2.50 | 3.50 | 58.14 | 62 ±2.63 |
17 | 60.00 | 2.00 | 4.50 | 49.44 | 54 ±2.45 |
18 | 50.00 | 2.50 | 3.50 | 58.14 | 60 ±1.38 |
19 | 60.00 | 3.00 | 4.50 | 39.94 | 42 ±1.33 |
20 | 50.00 | 2.50 | 3.50 | 58.14 | 58 ±1.23 |