The changes in BS and plasma amino acids in Ins2+/+ and Ins2+/−. Line graph for the change in the fasting BS and representative plasma amino acids from 3 to 20 weeks of age. To compare the change in the fasting BS and plasma amino acids between the Ins2+/− and Ins2+/+ mice, statistical analysis was performed using two-way ANOVA, and Bonferroni correction was used for the post hoc test. Data are the mean ± standard error of the mean. The solid line indicates Ins2+/− (n = 9–13). The dotted line indicates Ins2+/+ (n = 8–12). * p < 0.05, ** p < 0.01, Ins2+/− vs. Ins2+/+. This figure was revised based on our previous study (Mochida et al. 2011).