Figure 2

Administration of LFK suppresses the leukocytes infiltration into the lungs after PR8 viral infection. (A) Histology of lung tissue section stained with HE at DPI-7 (left: non-infected control, middle: saline-administered mice at DPI-7, right: LFK-administered mice at DPI-7). Original magnification is X10. Scale bars indicate 100 μm. (B) The lung cells were isolated at DPI-0, DPI-3, DPI-5, DPI-7 and DPI-10, and the absolute number of lung cells was counted (*: P < 0.01, **: P < 0.05, Student’s t test). Blue and red squares indicate saline- and LFK-administered groups, respectively (DPI-0: saline n = 7, LFK n = 7; DPI-3: saline n = 5, LFK n = 4; DPI-5: saline n = 5, LFK n = 4; DPI-7: saline n = 5, LFK n = 5; DPI-10: saline n = 3, LFK n = 5). (C) The change in lung cell population (blue: saline-administered group, red: LFK-administered group) was analyzed through a flow cytometer (DPI-0: saline n = 7, LFK n = 7; DPI-3: saline n = 5, LFK n = 4; DPI-5: saline n = 5, LFK n = 4; DPI-7: saline n = 5, LFK n = 5; DPI-10: saline n = 3, LFK n = 5). (D) The total number of BALF cells at DPI-5 was counted (blue: saline-administered group, n = 8; red: LFK-administered group, n = 6; *, P < 0.05, Student’s t test). (E) Cell population in BALF was analyzed through a flow cytometer (blue: saline-administered group, n = 8; red: LFK-administered group, n = 6).