Figure 4From: Characterisation of enterovirus 71 replication kinetics in human colorectal cell line, HT29 Expression SCARB2 in RD and HT29 cells. Total intracellular RNA were harvested from RD and HT29 cells, converted to cDNA and measured by quantitative real time polymerase chain reaction (qRT-PCR) with primers specific to SCARB2 and ACT. Primers for SCARB2 and ACT were designed to span exon-exon boundaries to give a single PCR product of 89Â bp and 198Â bp respectively. The presence of SCARB2 in HT29 further supports HT29 cells as a viable in vitro model to study EV71 pathogenesis.Back to article page