Skip to main content
Figure 3 | SpringerPlus

Figure 3

From: A simple set of validation steps identifies and removes false results in a sandwich enzyme-linked immunosorbent assay caused by anti-animal IgG antibodies in plasma from arthritis patients

Figure 3

Testing for false negative results and other matrix effects. (A) False negative measurements. OD signal from unspiked and spiked plasma samples from two arthritis patients in wells coated with anti-IL-24 specific antibody. (B) Identification and removal of false negative measurements. OD signal from unspiked and spiked plasma samples from the same two arthritis patients in wells coated with anti-IL-24 specific antibody after preincubating samples with a combination of mouse, goat, bovine and human immunoglobulins. (C) Spike recovery assessment in three other arthritis patients. Calculated IL-24 concentrations from unspiked patient samples and patient samples spiked with 800 pg/ml rh IL-24 in wells coated with anti-IL-24 specific antibody after preincubating samples with immunoglobulins. (D) Linearity-of-dilution assessment in three other arthritis patients. Calculated IL-24 concentrations in samples preincubated with immunoglobulin and diluted 4/6, 2/6 and 1/6 in wells coated with anti-IL-24 specific antibody.

Back to article page