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Figure 2 | SpringerPlus

Figure 2

From: A simple set of validation steps identifies and removes false results in a sandwich enzyme-linked immunosorbent assay caused by anti-animal IgG antibodies in plasma from arthritis patients

Figure 2

Testing for false positive results. (A) False positive measurements. OD signal from four patient samples in wells coated with anti-IL-24 specific antibody or with isotype control antibody. (B) Identification and removal of false positive measurements. OD signal from the same four patient samples in wells coated with anti-IL-24 specific antibody or with isotype control antibody after preincubating samples with a combination of mouse, goat, bovine and human immunoglobulins. (C) Identification of species specificity of the anti-animal IgG antibodies in the patient samples. OD signal from the same four patient samples in wells coated with isotype antibody after preincubating samples with different immunoglobulins as indicated.

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