Skip to main content
Figure 1 | SpringerPlus

Figure 1

From: A simple set of validation steps identifies and removes false results in a sandwich enzyme-linked immunosorbent assay caused by anti-animal IgG antibodies in plasma from arthritis patients

Figure 1

Preparing the sandwich ELISA system. (A) Testing different blockers of non-specific binding sites in polystyrene wells. OD signal from wells with blank assay diluent after blocking the wells with different blocking reagents. (B) Titration of capture and detection antibodies. OD signal from wells with blank assay diluent (noise) and wells with 1000 pg/ml rh IL-24 in assay diluent (signal) using different concentrations of capture antibody and detection antibody. Numbers represent concentrations in μg/ml. (C) OD signal from wells with blank assay diluent (noise) and wells with 1000 pg/ml rh IL-24 in assay diluent (signal) without or with amplification. (D) OD signal from wells with plasma samples from three different arthritis patients without or with amplification.

Back to article page