The impact of microcarrier culture optimization on the glycosylation profile of a monoclonal antibody

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Table 4 Conditions tested for the culture of monoclonal antibody-producing CHO-K1 cells in Cytodex 3 microcarriers

Assay	Inoculum concentration (cells/mL)	Rocking mechanism	Rocking speed (rpm)	Volume at inoculation	Culture vessel
CY1	2×10⁵	Pulse followed by continuous^a	60	Total	Vented conical tube
CY2	2×10⁵	Continuous	60	Total	Vented conical tube
CY3	2×10⁵	Pulse followed by continuous^a	40	Total	Vented conical tube
CY4	2×10⁵	Continuous	40	Total	Vented conical tube
CY5	2×10⁵	Pulse followed by continuous^a	60	Half	Vented conical tube
CY6	4×10⁵	Pulse followed by continuous^a	60	Total	Vented conical tube
CY7	4×10⁵	Continuous	60	Total	Vented conical tube
CYS	4x10⁵	Pulse followed by continuous^a	60	Total	Shake flask

^a Pulse rocking (60 or 40 rpm for 1 min at each 30 min) during the first six hours of culture, followed by continuous rocking for the remaining time of culture.