The impact of microcarrier culture optimization on the glycosylation profile of a monoclonal antibody

Costa, Ana Rita; Withers, Joanne; Rodrigues, Maria Elisa; McLoughlin, Niaobh; Henriques, Mariana; Oliveira, Rosário; Rudd, Pauline M; Azeredo, Joana

doi:10.1186/2193-1801-2-25

SpringerPlus

Table 3 Galactose, core fucose and sialic acid composition of the monoclonal antibody produced by CHO-K1 cells during microcarrier culture in different conditions: T-flask; CYi - Cytodex 3 in vented conical tubes; CYS - Cytodex 3 in shake flask

From: The impact of microcarrier culture optimization on the glycosylation profile of a monoclonal antibody

	% Area
Glycan structures	T-flask	CY1	CY2	CY3	CY4	CY5	CY6	CY7	CYS
Total peaks	17	17	20	19	19	17	20	15	11
Agalactosylated (G0)	46.67	29.35	23.64	28.35	30.41	34.06	25.16	36.56	39.70
Galactosylated (G1+G2+G3)	53.34	70.65	76.37	71.63	69.58	65.93	74.85	63.42	60.31
Monogalactosylated (G1)	36.11	48.69	38.59	39.64	42.85	45.34	40.65	43.06	49.74
Digalactosylated (G2)	14.06	20.48	27.45	23.02	21.13	18.25	26.29	18.97	10.57
Trigalactosylated (G3)	3.17	1.49	10.33	8.97	5.61	2.35	7.91	1.40	0.00
Core fucosylated (F)	89.01	78.74	72.06	73.63	73.36	54.90	72.19	88.89	71.71
Core fucosylated agalactosylated (FG0)	40.99	22.01	16.59	20.59	21.24	16.93	16.87	30.92	27.57
Core fucosylated monogalactosylated (FG1)	35.36	38.08	28.31	29.48	31.91	23.39	29.93	40.54	34.87
Core fucosylated digalactosylated (FG2)	10.33	17.16	20.55	17.71	16.48	12.23	20.31	16.03	9.27
Core fucosylated trigalactosylated (FG3)	2.33	1.49	6.61	5.85	3.73	2.35	5.08	1.40	0.00
Sialylated (S1+S2+S3)	10.73	8.18	27.14	23.07	15.78	14.07	22.77	7.75	1.56
Monosialylated (S1)	4.62	4.71	6.68	6.13	5.12	6.93	7.28	3.87	1.56
Disialylated (S2)	5.00	3.47	17.92	14.93	9.32	7.14	13.70	3.88	0.00
Trisialylated (S3)	1.11	0.00	2.54	2.01	1.34	0.00	1.79	0.00	0.00
Monosialylated monogalactosylated (S1G1)	2.73	2.16	2.18	1.91	1.83	2.86	2.54	1.72	1.56
Monosialylated digalactosylated (S1G2)	1.89	2.55	4.50	4.22	3.29	4.07	4.74	2.15	0.00
Monosialylated trigalactosylated (S1G3)	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00
Disialylated digalactosylated (S2G2)	2.94	1.98	10.13	7.97	5.05	4.79	7.58	2.48	0.00
Disialylated trigalactosylated (S2G3)	2.06	1.49	7.79	6.96	4.27	2.35	6.12	1.40	0.00
Trisialylated trigalactosylated (S3G3)	1.11	0.00	2.54	2.01	1.34	0.00	1.79	0.00	0.00
Complex glycans	98.43	97.74	98.09	97.81	97.62	96.81	97.76	97.87	97.30
High mannose	1.58	2.26	1.92	2.19	2.38	3.19	2.25	2.13	2.71

The major structures are marked in bold.

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