Figure 3

pAt5g17340:UidA:GFP induced GUS staining in qrt mutant pollen and during pollination. GUS staining is also detected in the germinating pollen grains (white arrowheads) and in the germinated pollen tube inside the style (black arrow) (a). GUS stained tetrads of tricellular pollen grains from the pAt5g17340:UidA:GFP / qrt1-1/qrt1-1 marker line (c) and tetrad of qrt1-1/qrt1-1 mutant tricellular pollen grains carrying no marker gene (e). DAPI staining (b, d) of the X-Gluc incubated pollen grains (c, e). GUS staining obtained after overnight incubation. Marker line plants: qrt1-1 and GC30 (with qrt1-1 pollen); G57 (pollination). Scale bars correspond to 40 μm.