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Figure 2 | SpringerPlus

Figure 2

From: A novel Arabidopsis marker line that strongly labels uninucleate microspores and the subsequent male gametophyte development stages

Figure 2

X-Gluc incubated Col-0 uninucleate microspores and pollen grains. No GUS staining (right column) is detected in late uninucleate microspores (Uni), nor in the bicellular (Bi) or tricellular (Tri) pollen grains of wild-type Col-0 landrace. The inflorescences were incubated overnight with X-Gluc substract to induce GUS staining. Plates show DAPI stained nuclei (left column) of the X-Gluc incubated (right column) microspores and pollen grains. Uni- uninucleate microspore; Bi- bicellular pollen; Tri- tricellular pollen. Col-0 plant. Scale bars correspond to 10 μm.

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