Table 2 List of oligonucleotides used for RT-PCR (h: human; m: murine; f: forward primer; r: reverse primer; TG: target gene; AT: annealing temperature)

Interferon α (human) - NM_024013.2

hIFN-α1

f:

acccacagcct ggataacag

60

r:

ctctcctcctgcatcacaca

Interferon β (human) - NM_002176.2

hIFN-β

f:

actgcctcaaggacaggatg

60

r:

agccaggaggttctcaacaa

Interleukin 1α (human) - NM_000575.3

hIL-1α

f:

aatgacgccctcaatcaaag

60

r:

tgggtatctcagqcatctcc

Interleukin 6 (human) - NM_000600.3

hlL-6

f:

caatgaggagacttgcctgg

63

r:

gcacagctctggcttgttcc

Interleukin 8 (human) - NM_000584.3

hIL-8

f:

tctgcagctctgtgtgaagg

63

r:

aatttctgtgttggcgcagt

Tumor necrosis factor α (human) - NM_000594.3

hTNFα

f:

aacctcctctctgccatcaa

62

r:

ggaagacccctcccagatag

18S ribosomal RNA (human) - X03205.1

hl8S

f:

gaaaatgcgaatggctcattaaa

60

r:

cacagttatccaagtaggagagg

Toll-like receptor 3 (human) - NM_003265.2

hTLR-3

f:

agccttcaacgactgatgct

60

r:

tttccagagccgtgcta.agt

Toll-like receptor 7 (human) - NM_016562.3

hTLR-7

f:

ccacaaccaactqaccactg

60

r:

ccacca.gacaaaccacacag

Toll-like receptor 8 (human) - NM_138636.4

hTLR-8

f:

gtttcctcgtctcgagttgc

60

r:

tcaaaggggtttccgtgtag

Toll-like receptor 9 (human) - NM_017442.3

hTLR-9

f:

cctattcatggacggca.act

60

r:

gagtgacaggtgggtgaggt

Myeloid Differentiation Primary Response Gene 88 (human) - NM 001172567.1

hMyD88

f:

tgcagagcaaggaatgtgac

60

r:

aggatgctggggaactcttt

Interferon Regulatory Factor 3 (human) - NM 001571.5

hIRF-3

f:

qaggtgacagccttctaccg

60

r:

tgcctcacgtagctcatcac

Interferon Regulatory Factor 7 (human) - NM 001572.3

hIRF-7

f:

taccatctacctgggcttcg

60

r:

gctccataaggaagcactcg

Melanoma Differentiation-Associated Gene 5 (human) - NM_020746.4

hMda-5

f:

ggggcatggagaataactca

60

r:

tgcccatgttgctgttatgt

NACHT, LRR and PYD Domains-Containing Protein 3 (human) - NM 004895.4

hNALP3

f:

cttctctgatgaggcccaag

60

r:

gcagcaaactggaaaggaag

Retinoic Acid inducible Gene l(human) - NM 014314.3

hRIG-I

f:

gcaacagtgcagaggtgaaa

60

r:

caaaagagcatccagcaaca

Interferon α (murin) - NM_010502.2

mIFN-α

f:

tcaatgacctgcaagctgtc

62

r:

agcaattggcagaggaagac

Interferon β (murin) - NM_010510.1

mIFN-β

f:

ccctatggagatgacggaga

60

r:

ctgtctgctggtggagttca

Interleukin 1α (murin) - NM_010554.4

mIL-1α

f:

gcaacgggaagattctgaag

62

r:

tgacaaacttctgcctgacg

Interleukin 6 (murin) - NM_031168.1

mIL 6

f:

ccggagaggagacttcacag

60

r:

tccacgatttcccagagaac

Tumor necrosis factor α (murin) - NM_013693.2

mTNFα

f:

ccgatgggttgtaccttgtc

60

r:

cggactccgcaaagtctaag

18S ribosomal RNA (murin) - NR_003278.3

m18S

f:

cgcggttctattttgttggt

60

r:

agtcggcatcgtttatggtc

Green Fluorescent Protein - L29346.1

GFP

f:

acgtaaacggccacaagttc

60

r:

aagtcgtgctgcttcatgtg

DNA- Dependent Activator of Interferon- Regulatory Factors (human) - NM 030776.2

hDAI

f:

aaag catggacgattta ccg

60

r:

atgatgttcccgtgtccaat

Interferon, gamma- inducible Protein 16 (human) - NM 001206567.1

hIFI16

f:

gctgaccgaaacatggagat

60

r:

cagatctcaactccccggta

Interleukin 1β (human) - NM_000576.2

hIL-1β

f:

ttcgacacatgggataacga

60

r:

tctttcaacacgcaggacag

TIR- Domain-containing Adapter-inducing Interferon-β (human) - AB093555.1

hTRIF

f:

caggagcctgaggagatgag

60

r:

ctgggtagttggtgctggtt

Interferon-beta Promoter Stimulator 1 (human) - AB232371.1

hIPS-1

f:

ataagtccgagggcaccttt

60

r:

gtgactaccagcacccctgt

Abscent In Melanoma 2(human) - NM_004833.1

hAM2

f:

gctgcaccaaaagtctctcc

60

r:

tcaaacgtgaagggcttctt

Caspase-l (human) - NM_033292.3

hCASP-1

f:

gaaggcatttgtgggaagaa

60

r:

ggtgtggaagagcagaaagc